The TRANSIL albumin binding kits are designed to assess a drug candidate’s tendency to bind to serum albumin and thus indicates how freely it is available in the blood. The kits are available with human serum albumin (HSA) or rat serum albumin (RSA). Both TRANSIL HSA Binding and TRANSIL RSA Binding kits are available in a ready-to-use format that requires an incubation time of only two minutes.
The TRANSIL AGP Binding kit is designed to assess the tendency of a drug candidate to bind to human α1-acid glycoprotein. Binding data from this assay yield clearly defined and highly reliable estimates of full plasma protein binding when used in combination with our TRANSIL HSA Binding kit.
The TRANSILXL High Sensitivity Binding Kit accurately determines the unbound fraction of drugs that are tightly bound to plasma proteins - even when the unbound fraction is well below 1%. Also, the TRANSIL assay has exellent recovery for drugs that exhibit too high unspecific binding in other assay systems, or precipitate because of deficient solubility. It determines the fraction of drug bound to plasma indirectly by measuring the partitioning of drug between the plasma proteins and artificial cell membranes. The kit consists of silanized glass vials pre-filled with buffer and membrane beads. It requires only 6 steps: (i) addition of plasma, (ii) addition of drug candidate, (iii) incubation for 30 minutes while mixing, (iv) removal of beads by centrifugation, (v) precipitation of plasma, and (vi) quantification of compounds.
Advantages of TRANSIL:
To order your protein binding kits contact orders@sovicell.com.
